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KMID : 0380020100250060520
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 6 p.520 ~ p.526
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Immobilization of Bacillus sp. Strains, Catalase Producing Bacteria and Their Hydrogen Peroxide Removal Characteristics
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Han Kyung-Ah
Jang Yun-Hee
Lee Jong-Il
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Abstract
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In this work we have investigated the production of catalase from Bacillus sp. strains, which were screened and identified from soil. These strains were cultivated in shaking flasks with tryptic soy broth (TSB) at 30℃ and 200 rpm. Effects of the temperature and pH on the stability of the native catalase and whole cell viability were studied in the temperature range of 25-60℃ and the pH range of 7-13. Korean natural zeolite was added to culture medium and mixed with microorganisms for 24 hours. The native catalase maintained its activity over 50℃. The enzyme acitiviy of the catalase from Bacillus flexus BKBChE-3 was highest among the Bacillus sp. strains studied. Bacillus flexus BKBChE-3 and immobilized Bacillus cells have survived under extreme conditions of over 50℃ and pH 12. 60 mL of 10.5 mM H2O2 solution were entirely removed within 1 hour with catalase produced from Bacillus sp. on the flask. When Bacillus cells were immobilized on Korean natural zeolite, colony forming unit of Bacillus flexus BKBChE-3 was increased and high efficiency of hydrogen peroxide removal was observed.
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KEYWORD
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Catalase, Hydrogen peroxide, Bacillus sp., Immobilization, Zeolite
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